Long-Term Human Hematopoietic Stem Cell Culture in Microdroplets

We previously reported a new approach for micromanipulation and encapsulation of human stem cells using droplet-based microfluidic device. This demonstrated the possibility encapsulating culturing difficult-to-preserve primary hematopoietic an engineered double-layered bead composed by inner layer alginate outer Puramatrix. also maintenance expansion Multiple Myeloma in this construction. Here, presented technique is applied to construct 3D biomimetic model recapitulate cell niche hydrogel beads cultured 10% FBS culture medium. In model, long-term number hHSCS encapsulated proposed structures was observed. Additionally, phenotypic characterization generated performed order assess their stemness maintenance. Results indicate that ex vivo CD34+ from bone marrow were viable, maintained, expanded over time span eight weeks. novel methodology could represent breakthrough improve Hematopoietic Progenitor Transplant (HPT) as well tool further study biochemical biophysical factors influencing behavior. technology opens myriad applications universal potentially able expand other types cells.